000			02756cam a2200385Mi 4500
001			on1108525614
003			OCoLC
005			20240726104811.0
008			190713s2019 gw o 000 0 eng d
040			_aEBLCP _beng _epn _erda _cEBLCP _dOCLCQ _dYDX _dNT _dSNM
020			_a3736960468
020			_a9783736960466 _q((electronic)l(electronic)ctronic)
050	0	4	_aQK45 _b.P467 2019
049			_aMAIN
100	1		_aAhmad, Fatima Haj. _e1
245	1	0	_aPhosphoproteomics Analysis of the Systemin Signaling Pathway in tomato.
260			_aGöttingen : _bCuvillier Verlag, _c(c)2019.
300			_a1 online resource (191 pages)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
347			_adata file _2rda
490	1		_aSchriftenreihe zur Physiologie und Biochemie der Pflanzen ; _vv. 12
504			_a2
520	0		_aSystemin is a small peptide with important functions in plant wound response signaling. To elucidate systemin perception and signal transduction mechanisms, a phosphoproteomic profiling study was performed to reconstruct a systemin-specific kinase/phosphatase signaling network. Time course analyses revealed early events at the plasma membrane, such as dephosphorylation of H+-ATPase, and the phosphorylation of NADPH-oxidase and Ca2+-ATPase in response to systemin. Later responses included transient phosphorylation of small GTPases and vesicle trafficking proteins, as well as transcription factors. Based on a correlation analysis of systemin-induced phosphorylation profiles, substrate candidates for 44 systemin-responsive kinases and 9 phosphatases were predicted, some of which are involved in a regulatory circuit for the regulation of the plasma membrane H+-ATPase. In this regulatory model, H+-ATPase LHA1 is rapidly de-phosphorylated at its C-terminal regulatory residue T955 by phosphatase PLL5, resulting in the alkalization of the growth medium within two minutes of systemin treatment. LHA1 is re-activated by MAP-Kinase MPK2 later in the systemin response. A valuable resource of proteomic events involved in the systemin signaling cascade is provided with a focus on the prediction of substrates to early systemin-responsive kinases and phosphatases.
530			_a2 _ub
650		0	_aPlants _xWounds and injuries.
650		0	_aTomatoes.
650		0	_aPeptides.
655		1	_aElectronic Books.
856	4	0	_zClick to access digital title | log in using your CIU ID number and my.ciu.edu password. _uhttpss://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=2182773&site=eds-live&custid=s3260518
942			_cOB _D _eEB _hQK.. _m2019 _QOL _R _x _8NFIC _2LOC
994			_a92 _bNT
999			_c78711 _d78711
902			_a1 _bCynthia Snell _c1 _dCynthia Snell