Cover -- Title -- Copyright -- End User License Agreement -- Contents -- Foreword -- Preface -- DISCLAIMER -- FUNDING -- CONSENT FOR PUBLICATION -- CONFLICT OF INTEREST -- ACKNOWLEDGEMENT -- Abstract -- Introduction -- 1. OSTEOSARCOMA -- 1.1. Pathophysiology -- 1.1.1. Genetic Alterations -- 1.2. Diagnosis and Treatment -- 1.2.1. Targeted Therapy -- 1.2. Challenges to Osteosarcoma Treatment -- 1.2.1. Multidrug Resistance -- 1.2.2. Active Targeting -- 2. RNA INTERFERENCE TECHNOLOGY -- 2.1. miRNAs -- 2.1. miRNA-145 -- 3. GENE THERAPY -- 3.1. Viral and Non-viral Vectors 3.1.1. Polymeric Micelles -- 3.1.2. Cationic Polymers -- 3.1.3. Poloxamers and Poloxamines -- 3.1.4. Micelleplexes -- 3.1.5. Physicochemical Characterization -- AIMS -- Materials and Methods -- 1. MATERIALS -- 2. CHITOSAN COMPLEXED WITH PLURONIC® F68 -- 3. CHITOSAN COMPLEXED WITH PLURONICS® F68 AND P123 -- 4. SYNTHESIS OF PLURONIC® F68 DIACRYLATE -- 5. SYNTHESIS OF PLURONIC® F68 DIACRYLATE CONJUGATED WITH PEI (F68-PEI) -- 6. CHARACTERIZATION OF THE PREPARED POLYMERIC MICELLES -- 6.1. Evaluation of Particle Size and Zeta Potential -- 6.2. 1H-NMR Spectroscopy 6.3. Fourier-Transform Infrared Spectroscopy -- 6.4. Transmission Electron Microscopy -- 6.5. Statistical Analysis -- Results and Discussion -- 1. CHITOSAN COMPLEXED WITH PLURONIC® F68 -- 2. CHITOSAN COMPLEXED WITH PLURONICS® F68 AND P123 -- 3. SYNTHESIS OF PLURONIC® F68 DIACRYLATE -- 4. SYNTHESIS OF PLURONIC® F68 DIACRYLATE CONJUGATED WITH PEI -- 5. PREPATION OF MIXED PLURONIC® F68-PEI AND PLURONIC® P123 MICELLES -- 6. MORPHOLOGICAL CHARACTERIZATION -- Conclusion -- FUTURE PERSPECTIVES -- Annex -- 1. CHITOSAN COMPLEXED WITH PLURONIC® F68 2. SYNTHESIS OF PLURONIC® F68 DIACRYLATE CONJUGATED WITH PEI -- ABBREVIATIONS -- LIST OF ABBREVIATIONS -- REFERENCES -- REFERENCES -- Subject Index -- Back Cover
Osteosarcoma is a rare bone tumor that has a high incidence among children and young adults. Despite recent therapeutic developments, osteosarcomastill presents major hurdles to achieving successful results, mainly due to the presence of multi-drug-resistant cells.This monograph primarily aims to provide information about the basic science behind the treatment of osteosarcoma along with experimental results for a novel formulation that overcomes multidrug resistance, and therefore, may serve as a viable treatment option. The book starts with an updated and conciseguide to the pathophysiology of the.