TY  - BOOK
AU  - Ahmad,Fatima Haj
TI  - Phosphoproteomics Analysis of the Systemin Signaling Pathway in tomato
T2  - Schriftenreihe zur Physiologie und Biochemie der Pflanzen
SN  - 3736960468
AV  - QK45 .P467 2019
PY  - 2019///
CY  - Göttingen
PB  - Cuvillier Verlag
KW  - Plants
KW  - Wounds and injuries
KW  - Tomatoes
KW  - Peptides
KW  - Electronic Books
N1  - 2; 2; b
N2  - Systemin is a small peptide with important functions in plant wound response signaling. To elucidate systemin perception and signal transduction mechanisms, a phosphoproteomic profiling study was performed to reconstruct a systemin-specific kinase/phosphatase signaling network. Time course analyses revealed early events at the plasma membrane, such as dephosphorylation of H+-ATPase, and the phosphorylation of NADPH-oxidase and Ca2+-ATPase in response to systemin. Later responses included transient phosphorylation of small GTPases and vesicle trafficking proteins, as well as transcription factors. Based on a correlation analysis of systemin-induced phosphorylation profiles, substrate candidates for 44 systemin-responsive kinases and 9 phosphatases were predicted, some of which are involved in a regulatory circuit for the regulation of the plasma membrane H+-ATPase. In this regulatory model, H+-ATPase LHA1 is rapidly de-phosphorylated at its C-terminal regulatory residue T955 by phosphatase PLL5, resulting in the alkalization of the growth medium within two minutes of systemin treatment. LHA1 is re-activated by MAP-Kinase MPK2 later in the systemin response. A valuable resource of proteomic events involved in the systemin signaling cascade is provided with a focus on the prediction of substrates to early systemin-responsive kinases and phosphatases
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