Industrial biotechnology : priniciples and applications / Loveleen Kaur and Robinka Khajuria.

Includes bibliographies and index.

INDUSTRIAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS; INDUSTRIAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS; Library of Congress Cataloging-in-Publication Data; Contents; Preface; Section I: Principles of Industrial Biotechnology; Chapter 1: Industrial Biotechnology: Introduction and History; Products of Industrial Biotechnology; History; Fermentation Mysteries; The Emergence of Microbiology As a Science; Future of Biotechnology Industry; References; Chapter 2: Isolation and Screening of Industrially Important Microorganisms; Selection of Industrially Important Microorganisms.

Selection of Microorgamisms1. Chemical Methods; (a) Enrichment Culture Method; b) Use of minimal nutrient levels; c) Use of Inhibitors; 2. Physical Methods; (a) Heat; (b) Incubation Temperature; (c) pH; 3. Biological Methods; Pure Culture; Techniques for Isolation of Pure Culture; 1. Spread Plate Method; 2. Pour Plate Method; 3. Streak Plate Method; 4. Serial Dilution Method; Special Methods of Isolation of Pure Culture; 1. Single Cell Isolation Methods; 2. Culture Independent Molecular Techniques for Monitoring Complex Microbial Communities; 3. Novel Methods for Isolating Microorganisms.

Screening of MicroorganismsPrimary Screening; 1. Primary Screening for Organic Acid/ Amine Producers; 2. Primary Screening of Antibiotic Producers; (iii) Primary Screening of Growth Factor; Secondary Screening; Maintenance and Preservation of Pure Cultures; Refrigeration; Paraffin Method/ Mineral Oil Storage; Cryopreservation; Lyophilization; Preservation Using Silica Gel; Preservation on Paper; Preservation on Beads; Preservation on Soil; Culture Collection Banks; Strain Improvement; Mutation; (i) Mutants Producing Increased Quantities of Metabolites; (ii) Auxotrophic Mutants.

(Iii) Antimetabolite-Resistant Mutants(iv) Mutants Resistant to Nutritional Repression; (v) Permeability Mutants; (vi) Mutants Producing New Antibiotics; Genetic Recombination; Protoplast Fusion; Improvement of Microbial Processes by Genetic Engineering; Novel Genetic Technologies; References; Chapter 3: Media Design; Nutritional Requirements of Microorganisms; Growth Media; Types of Media; 1. Based on Consistency: Solid, Semisolid and Liquid Media; 2. Based on Composition: Defined and Undefined Media; 3. Special Purpose Media; Role of Media in Fermentation; Media Preparation.

Properties of MediaMedium Components; Carbon sources; Nitrogen Sources; Water; Minerals; Vitamins and Growth Factors; Precursors; Inducers and Elicitors; Inhibitors; Buffers; Cell Permeability Modifiers; AntiFoams; Oxygen; Medium Optimization; Media Sterilization; Methods of Sterilization; Thermal Resistance of Microorganisms; Thermal-Death Relationships of Bacterial Spores; Batch Sterilization; Continuous Sterilization; Media Economics; Media Components; Labor Costs; Contamination and Sterilization; Yield and Product Recovery; Product Purity; Waste Disposal; Research Costs; References.

Industrial biotechnology can be defined as the use of modern biological life sciences in various industries. Biotechnology has a myriad of applications in our day to day life such as with simple processes such as the brewing of beer, use of enzymes in detergents, production of fermented food, production of antibiotics, nutritional supplements et cetera This book also includes processes (production of biofuels, treatment of effluents) that contribute to creating efficient, eco-friendly environments. This book discusses the different aspects of bioprocesses; media design, fermenter design and the eco.

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